Experienced Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

91. D.pneumoniae can be identified by
a. Microscopic exam
b. Culture of sputum/blood
c. Animal inoculation
d. All of these
e. None of these
Ans: E

92. The diagnosis of tuberculosis is carried out by
a. Emulator
b. Antiformin method
c. Petroff’s method
d. Concentration method
e. All of these
Ans: E

93. The size of the virus can be determined by
a. Micrography
b. Ultra-centrifugation at high speed
c. Ultra-filteration
d. All of these
Ans: D

94. Differential staining of bacteria spore is related to
a. Albert’s staining
b. Lugol’ s staining
c. Moller’s staining
d. Indian ink preparation
Ans: C

95. Electron microscope studies does not help in identifying the section of bacterial spore
a. Core
b. Spore cortex
c. Capsule
d. All of these
Ans: C

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Freshers Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

81. Clsotridium welchii is positive for
a. Elek’s gel precipitation test
b. Nagler’s test
c. Weil felix test
d. Bacitracin test
Ans: B

82. Nagler’s reaction detects
a. Coagulase
b. Hyaluronidase
c. Lecithinase
d. None of these
Ans: C

83. Incubation period of Cl. welchii is
a. 8-12 hours
b. 7-10 hours
c. 5-7 hours
d. 2-4 hours
Ans: A

84. The average incubation period of tetanus is
a. 2-3 days
b. 7-10 days
c. 14-21 days
d. 3-4 weeks
Ans: B

85. Salt agar is used for
a. Streptococcus
b. Staphylococcus
c. Vibrio
d. Shigella
Ans: B

86. Culture medium of Leishmania is
a. Sabousand’s medium
b. NNN medium
c. Wilson Blair medium
d. Czapek – dox medium
Ans: B

87. A simple asexual spore which develops by budding is known as
a. Chlamydospore
b. Blastospore
c. Arthospore
d. Conidia
Ans: B

88. Culture medium used for fungus is
a. Sabouraud’s medium
b. Nutrient agar
c. Nutrient broth
d. Minimal agar medium
Ans: B

89. For sterilization of fermentation equipment the method followed is
a. Radiation
b. Chemicals
c. Heating
d. All of these
Ans: D

90. D.pneumoniae can be cultivated in
a. Glucose broth
b. Serum broth
c. Agar and blood agar
d. Chocolate agar
e. All of these
Ans: E

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Frequently Asked Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

71. Wilson and Blair medium is used for isolation of
a. Staphylococci
b. Salmonella typhosa
c. Vibrio cholerae
d. Shigella shigae
Ans: B

72. Laboratory diagnosis of enteric fever is based on
a. Blood culture
b. Urine and stool culture
c. Widal test
d. All of the above
Ans: D

73. Shigella was first isolated by
a. Shiga
b. Schmitz
c. Sonnei
d. Robert Koch
Ans: C

74. Which of the following are gas producing Salmonella?
a. S.typhi
b. S.enteritidis
c. S.cholerasuis
d. S.typhimurium
Ans: B

75. Kauffmann white scheme is used to detect
a. Salmonella spp.
b. Shigella spp.
c. E.coli
d. None of these
Ans: A

76. On Mac Conkey’s medium Esch. Coli forms
a. Colorless colonies
b. Greenish pigmentation
c. Pink coloured colonies
d. Medusa head appearance
Ans: C

77. C.diphtheriae requires
a. LJ medium
b. Mac Conkey’s medium
c. Potassium tellurite medium
d. PDA medium
Ans: C

78. Culture medium for Mycobacterium tuberculosis
a. L J medium
b. Mac Conkey’s medium
c. Wilson blair medium
d. None of these
Ans: A

79. Lepra bacillus is best cultured on
a. Armadillo’s brain
b. Foot pad of mice
c. Liver of guinea pig
d. Any of the above
Ans: B

80. Culture medium for clostridia spp.
a. 76 Lower stein Jensen’s medium
b. Mac Conkey’s medium
c. Robertson’s cooked meat medium
d. None of these
Ans: C

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Latest Sterilisation, Culture Media & Pure Culture Techniques Objective Type Questions And Answers

61. Salts of heavy metals used as disinfectants are
a. Thiomersal
b. Phenyl mercury nitrate
c. Mercurochrome
d. All of these
Ans: D

62. Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are
a. Stock cultures
b. Stabcultures
c. Sub-cultures
d. None of these
Ans: B

63. The principle involved in the streak plate method is
a. Separation
b. Streaking
c. Isolation
d. Dilution
Ans: D

64. Culture media for fungi are
a. Potato dextrose agar (PDA)
b. Sabouraud’s agar
c. Czapekdox agar
d. All of the above
Ans: D

65. Spores of actinomycetes are very sensitive, killed at room temperature of
a. 52oC for 30 min.
b. 65oC for 30 min.
c. 70oC for 30 min.
d. 43oC for 30 min.
Ans: B

66. The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures
a. Thermophiles
b. Extreme thermophiles
c. Thermoduric
d. Facultative thermophiles
Ans: C

67. A common laboratory method of cultivating anaerobic micro-organisms is
a. Gas pack system
b. Brewer jar system
c. Pyrogallic acid over the cotton
d. None of these
Ans: C

68. Alkaliphiles grow at pH value between
a. 1 to 6
b. 6 to 9
c. 1 to 11
d. 7 to 12
Ans: D

69. The micro-organisms grow at high salinity are
a. Osmophiles
b. Halophiles
c. Both a and b
d. None of these
Ans: C

70. Non-lactose fermenting colonies seen on Mac Conkey’s medium are
a. Salmonella typhi
b. Escherichia coli
c. Klebsiella pneumoniae
d. Shigella shigae
Ans: A

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Most Recently Asked Sterilisation, Culture Media & Pure Culture Techniques Objective Type Questions And Answers

51. The bacterial culture prepared by pure culture is
a. Inoculum
b. Suspension
c. Dilution
d. None of these
Ans: A

52. Separation of a single colony is
a. Pure-culturing
b. Isolation
c. Separation
d. Both a and b
Ans: B

53. Growth period of the culture is
a. Inoculation
b. Incubation
c. Incineration
d. Isolation
Ans: B

54. At the temperature 160oC for one hour, complete sterilization occurs in
a. Autoclave
b. Hot air oven
c. Laminar flow
d. Incubator
Ans: B

55. In autoclave, the principle involved is
a. Dry heat
b. Moist heat
c. Steam under pressur
d. Both b and c
Ans: D

56. The spores of th bacteria which can withstand the moist heat effect also
a. Bacillus subtilis
b. Coxiella burnetti
c. Bacillus stearothermophilus
d. Pseudomonas
Ans: C

57. Factors on which disinfectivity of a disinfectant depends
a. Concentration of the substance
b. Time of action
c. pH of the medium and temperature suitable for the chemical
d. All of the above
Ans: D

58. Aldehydes, which are most powerful disinfectants
a. Formaldehyde
b. Acetaldehyde
c. Glutamal aldehyde
d. Both a and c
Ans: D

59. Accridine dyes are more effective against
a. Gram positive
b. Gram negative
c. Mycoplasmas
d. Rickttsiae
Ans: A

60. The sterilizing agent is
a. Ethelene oxide
b. Oxygen
c. Nitrogen
d. Carbon tetrachloride
Ans: A

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Tricky Sterilisation, Culture Media & Pure Culture Techniques Objective Type Questions And Answers

41. Tyndallisation was proposed by
a. Tyndall
b. Pasteur
c. Koch
d. Jenner
Ans: A

42. Viruses can be cultivated in
a. Lab media
b. Broth
c. Living cells
d. None of these
Ans: C

43. By pasteurization
a. All the microorganisms can be removed
b. Only pathogenic forms can be removed
c. Only non-pathogenic forms can be removed
d. All of these are correct
Ans: B

44. The temperature required for pasteurization is
a. Above 100oC
b. Below 100oC
c. 100oC
d. None of these
Ans: B

45. In the medium other than nutrients, if any substance is used in excess, that medium is
a. Enriched medium
b. Special medium
c. Enrichment medium
d. None of these
Ans: A

46. Example for indicator medium is
a. Nutrient Agar
b. Nutrient broth
c. Wilson and Blair
d. Czapeck-dox medium
Ans: C

47. Example of Anaerobic medium is
a. Robertson cooked-meat medium
b. Nutrient agar
c. Nutrient broth
d. Mac-Conkey’s agar
Ans: A

48. The differentiate lactose and non-lactose fermentors, the medium used is
a. Wilson & lair
b. Blood Agar
c. Tetra thionate broth
d. Mac-Conkey’s Agar
Ans: D

49. Best method for getting pure culture is
a. Streak-plate
b. Agar slant
c. Both a & b
d. None of these
Ans: C

50. To transfer cultures from one place to another, the device used is
a. Slant
b. Needle
c. Inoculation loop
d. Autoclave
Ans: B

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Basic Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

31. Lyophilization means
a. Sterilization
b. Freeze-drying
c. Burning to ashes
d. Exposure to formation
Ans: B

32. Temperature used for hot air oven is
a. 100oC for 1 hour
b. 120oC for 1 hour
c. 160oC for 1 hour
d. 60oC for 1 hour
Ans: C

33. Phenol co-efficient indicates
a. Efficiency of a disinfectant
b. Dilution of a disinfectant
c. Purity of a disinfectant
d. Quantity of a disinfectant
Ans: A

34. This is an agar plate method and is commonly used for estimation of the number of bacteria in milk.
a. Standard Plate Count (SPC)
b. Spread plate
c. Lawn culture
d. Roll tube method
Ans: A

35. Agar is obtained form
a. Brown algae
b. Red algae
c. Green algae
d. Blue-green algae
Ans: B

36. A gram positive organism which produces swarming on culture medium is
a. Salmonella
b. Clostridium
c. Staphylococci
d. Proteus
Ans: D

37. Enhancement of virulence in bacteria is known as
a. Pathogenicity
b. Attenuation
c. Exaltation
d. Toxigenicity
Ans: C

38. For effective sterilization in an autoclave the temperature obtained is
a. 50oC
b. 100oC
c. 120oC
d. 180oC
Ans: C

39. Spores are killed by
a. 70% alcohol
b. Glutaraldehyde
c. Autoclaving
d. Both b and c
Ans: D

40. Glassware are sterilized by
a. Autoclaving
b. Hot air over
c. Incineration
d. None of these
Ans: B

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Common Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

21. L-Lysine is produced from
a. Corynebacterium glutamicum
b. Clostridium botulinum
c. Mycobacterium sps
d. Pseudomonas
Ans: A

22. The orderly increase in the quantity of all of the cellular components is known as
a. Reproduction
b. Growth
c. Binary fission
d. None of these
Ans: B

23. Theobacillus thio oxidans grow at pH
a. 7.0
b. 1.0
c. 6.0
d. 9.5
Ans: B

24. Slow freezing requires the conditions
a. 0oC to 15oC for 15 min.
b. – 6 oC to – 10oC for 10 min.
c. – 15oC to 3 to 72 hrs.
d. None of these
Ans: C

25. Discontinuous heating is called
a. Pasteurization
b. Sterilization
c. Fermentation
d. Tindalisation
Ans: D

26. Isolation is
a. Purification of culture
b. Introduction of inoculum
c. Separation of a single colony
d. To grow microorganisms on surfaces
Ans: C

27. The condition required for autoclave
a. 121oC temp.and 15 lbs. pressure for 20 min.
b. 120oC temp.and 20 lbs. pressure for 30 min
c. 150oC temp.for 1 hr.
d. 130oC temp for 2 hr.
Ans: C

28. Lysozyme is effective against
a. Gram negative bacteria
b. Gram positive bacteria
c. Protozoa
d. Helminthes
Ans: B

29. Blood agar medium is
a. Enrichment medium
b. Enriched medium
c. Selective medium
d. Differential medium
Ans: B

30. Infrared radiation is a method of sterilization by
a. Dry heat
b. Moist heat
c. Chemical method
d. Mechanical method
Ans: D

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