Monday 26 October 2015

Molecular Biology Objective Type Questions And Answers

6- The Polymerase Chain Reaction (PCR) is used to amplify small amounts of DNA for further analysis. First the DNA double helix must be split into two strands. This is achieved by
1) alkali solution
2) centrifugation
3) DNA polymerase
4) heating to nearly 100°C
5) viral reverse transcriptase
Answers-4

7- Protein synthesis occurs within cells. A particular molecule that is produced in the nucleus initiates protein synthesis. This molecule matures in the cytoplasm and binds to the ribosome. This molecule is
1) messenger RNA
2) ribosomal RNA
3) RNA nucleotide
4) RNA polymerase
5) transfer RNA
Answers-1

8- Northern blotting is a technique that can be used to detect:
1) Antibodies
2) DNA
3) RNA
4) Protein
5) Plasmids
Answers-3
Northern blotting is a means of detecting RNA, frequently used to quantify specific mRNA transcript levels.

9- Which of the following statements regarding messenger RNA (mRNA) is correct?
1) mRNA never contains introns.
2) mRNA is translated into proteins in the nucleus.
3) mRNA contains the bases cytosine and thymine.
4) reverse transcriptase uses mRNA as a template to produce complementary DNA.
5) mRNA is used in the Southern blotting technique.
Answers-4

10- Transcription RNA (tRNA) has three bases specific for a particular amino acid with which it binds to messenger RNA (mRNA). This specific area of tRNA is called the
1) anticodon
2) codon
3) exon
4) intron
5) transposon
Answers-1

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